Cis-Acting Elements for Proper Ontogenic Expression of Arylsulfatase Gene of Sea Urchin Embryo
نویسندگان
چکیده
منابع مشابه
Distal cis-acting elements restrict expression of the CyIIIb actin gene in the aboral ectoderm of the sea urchin embryo
The distal region of the S. purpuratus actin CyIIIb gene, between -400 and -1400 nucleotides, contains at least three distinct cis-acting elements (C1R, C1L and E1) which are necessary for correct expression of fusion reporter genes in transgenic sea urchin embryos. The contribution of these elements in the temporal and spatial regulation of the gene was analyzed by single and double site-direc...
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Several cis-regulatory elements within the 5' regulatory region of the lineage-specific CyIIIa actin gene have been identified by in vivo competition. Sea urchin eggs were coinjected with a fusion construct in which the bacterial chloramphenicol acetyltransferase (CAT) gene is controlled by the CyIIIa regulatory domain, together with molar excesses of various DNA subfragments that are derived f...
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Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...
متن کاملOntogenic expression of a Cyl actin fusion gene injected into sea urchin eggs
The 5' terminus of the Cyl actin gene transcription unit of Strongylocentrotus purpuratus was located by primer extension and other procedures, and the flanking upstream region was partially sequenced and mapped. A fusion gene was constructed containing about 2-5 kb of 5' flanking sequence, the transcribed leader sequence, and the first few codons of the Cyl gene ligated to the bacterial gene c...
متن کاملModular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo.
The Endo16 gene of Strongylocentrotus purpuratus is expressed at the blastula stage of embryogenesis throughout the vegetal plate, at the gastrula stage in the whole of the archenteron and in postgastrular stages only in the midgut. We showed earlier that a 2300 bp upstream sequence suffices to faithfully recreate this pattern of expression when fused to a CAT reporter gene. Here we define the ...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1992
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1992.tb00041.x